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Amgen Scientists Publish New Tool to Aid Selection of rProtein Leads | BioQuality.biz

Productivity and Quality of Recombinant Proteins Produced by Stable CHO Cell Clones can be Predicted by Transient Expression in HEK Cells (click the large title above to see the whole post)


Mol Biotechnol. 2012 Aug 23. [Epub ahead of print], Diepenbruck C, Klinger M, Urbig T, Baeuerle P, Neef R., Amgen Research (Munich) GmbH, Staffelseestr. 2, 81477, Munich, Germany.


Bullet-point Overview:

  • The authors describe an approach that allows prediction of the productivity and quality of recombinant proteins by stable producer cell clones with the help of transient transfection studies
  • As examples, they presented results from three distinct bispecific T cell engager (BiTE(®))-a new class of single-chain antibody-based therapeutics showing very promising results in the treatment of cancer
  • They found that BiTE(®) titers of transiently transfected HEK cells showed a striking correlation with titers of selected stable CHO cell clones
  • In addition, the percentage of the monomeric BiTE(®) fraction in cell culture supernatants correlated well between transiently expressing HEK and stably expressing CHO cell clones
  • According to the authors, this validates the use of transient transfection studies for the selection of biopharmaceutical lead candidates with desired pharmaceutical properties


To see the Abstract, and a link to obtain this paper: http://www.ncbi.nlm.nih.gov/pubmed/22915356


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